01 Solution Summary
Extracts of grains, nuts, vegetable oils, and other foods requiring aflatoxin testing are separated by HPLC; the target analytes then react with derivatization reagent in the post-column flow path and are measured by a matched detector for aflatoxin B and G group components. Quantitation and quality control use the following arrangement: external standard calibration with aflatoxin standard solutions, with blanks, spike recovery, and calibration checks controlling extraction, cleanup, and derivatization response. Each analytical batch also includes blanks, calibration checks, and applicable QC samples.
02 Standards and Method Basis
Method basis: application method. The laboratory may enter the name and version of the adopted formal method into the project file.
03 Samples and Analytes
The specific samples areExtracts of grains, nuts, vegetable oils, and other foods requiring aflatoxin testing, and the analytes areAflatoxin B and G group components. Pretreatment, injection, and detection configurations are determined according to the sample state, target concentration, and the formal method adopted.
04 Method Principle
The sample is first separated on a liquid chromatography column; the effluent is then mixed online with the derivatization reagent and forms detectable products under controlled reaction conditions.
05 Sample Collection and Pretreatment
Extracts of grains, nuts, vegetable oils, and other foods requiring aflatoxin testing are subjected to liquid injection after extraction, cleanup, and filtration; the derivatization reagent is prepared separately and checked with a blank.
06 Separation and Detection
The LC pump, chromatography column, derivatization pump, mixer, reaction temperature control, and detector form a continuous flow path.
07 Instrument System Configuration
| Module | Configuration | Role in This Solution |
|---|---|---|
| HPLC separation | Pump, autosampler, and chromatography column | Separate target analytes first |
| Post-column derivatization | Derivatization pump, mixing, and reaction temperature control | Generate detectable products |
| Detection Unit | FLD | Acquire derivatization product response |
08 Huishi Instruments system configuration
This application requiresHigh-performance liquid chromatography and post-column derivatization systemComplete the analysis of aflatoxin B and G group components. When selecting a configuration, the injection interface, detector, and data system should be determined based on the sample state of extracts of grains, nuts, vegetable oils, and other foods requiring aflatoxin testing, the liquid autosampler, and detection requirements.
09 Analysis workflow
10 Qualitative, Quantitative, and Quality Control
Use external standard calibration with aflatoxin standard solutions, and use blanks, spiked recovery, and calibration checks to control extraction, cleanup, and derivatization response. Derivatization reagent blanks, calibration checks, and reaction stability are included in batch quality control.
11 Method and Configuration Selection
- The derivatization module is located after chromatographic separation.
- Reaction time, temperature, and reagent flow rate are matched to detector response.
12 Frequently Asked Questions
Why are aflatoxin B- and G-group components analyzed by HPLC post-column derivatization?
HPLC post-column derivatization matches the physicochemical properties, sample introduction, and detection targets of aflatoxin B- and G-group components; the complete workflow covers the processing, separation, detection, and data quality control of cereals, nuts, vegetable oils, and other food extracts requiring aflatoxin testing.
What samples is this solution applicable to?
Applicable samples are cereals, nuts, vegetable oils, and other food extracts requiring aflatoxin testing. When the sample source or matrix changes, the effects of co-existing components on sample preparation, separation, and detection response should be re-evaluated.
What components or indicators are included in the detection targets?
The detection targets are aflatoxin B- and G-group components, and both the main text and configuration are bounded by this entity scope.
How is quality controlled for an analytical batch?
For each batch of samples, set up method blanks, calibration verification, duplicate samples, or applicable QC samples, and retain records of sample processing, instrument conditions, calibration, and calculations.
How are the sample introduction and detection modules matched in this solution?
The liquid autosampler is responsible for introducing processed cereals, nuts, vegetable oils, and other food extracts requiring aflatoxin testing into the analysis system, and the detection unit is configured for the response characteristics of aflatoxin B- and G-group components; both must be consistent with the method used.
13 Related Solutions and Knowledge
Get Configuration and Technical Consultation
Please provide sample information for cereals, nuts, vegetable oils, and other food extracts requiring aflatoxin testing, the concentration ranges of aflatoxin B- and G-group components, testing batch size, method basis, and existing equipment. Huishi Instruments will prepare a configuration list accordingly.
Information submitted.